Western blot using Rockland's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lane 1: mock-irradiated Drosophila melanogaster (3rd instar) larvae brain WC lysate. Lane 2: 4000-RAD gamma irradiated Drosophila melanogaster (3rd instar) larvae brain WC lysate. Separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-103-122) and ECL for detection.

Western blot using Rockland's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lane 1: mock-irradiated Drosophila melanogaster (3rd instar) larvae brain WC lysate. Lane 2: 4000-RAD gamma irradiated Drosophila melanogaster (3rd instar) larvae brain WC lysate. Separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-103-122) and ECL for detection. Personal Communication. Yikang Rong,

hth mutant nuclei show increased number of DNA breaks.A,A´) Wild type syncytium (cycle 11) stained for His2AvP (green) and topro3 (blue). Due to the lack of mitotic checkpoints during the rapid syncytial divisions the nuclei show some degree of DNA breaks marked with the anti-His2AvP antibody (arrowheads in A´). B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA. C) Quantification of the DNA breaks in the Dfhth mutant embryos and in their wild type siblings. Quantification was performed by counting the number of nuclear dots marked with the anti-His2AvP antibody. (N = 50 nuclei for each genotype from 5 different embryos each. P-value: 7,0085X10−5). Figure provided by CiteAb. Source: PLoS One, PMID: 25794008.

hth mutant nuclei show increased number of DNA breaks.A,A´) Wild type syncytium (cycle 11) stained for His2AvP (green) and topro3 (blue). Due to the lack of mitotic checkpoints during the rapid syncytial divisions the nuclei show some degree of DNA breaks marked with the anti-His2AvP antibody (arrowheads in A´). B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA. C) Quantification of the DNA breaks in the Dfhth mutant embryos and in their wild type siblings. Quantification was performed by counting the number of nuclear dots marked with the anti-His2AvP antibody. (N = 50 nuclei for each genotype from 5 different embryos each. P-value: 7,0085X10−5). Figure provided by CiteAb. Source: PLoS One, PMID: 25794008.